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DNMT1 antibody

DNMT1 antibody

Catalog Number: orb148083

DispatchUsually dispatched within 2-3 weeks
$ 575.00
Catalog Numberorb148083
CategoryAntibodies
DescriptionMouse monoclonal to DNMT1 (ATTO-390). Methylation of DNA at cytosine residues plays an important role in regulation of gene expression, genomic imprinting and is essential for mammalian development. Hypermethylation of CpG islands in tumor suppressor genes or hypomethylation of bulk genomic DNA may be linked with development of cancer. To date, 3 families of mammalian DNA methyltransferase genes have been identified which include Dnmt1, Dnmt2 and Dnmt3. Dnmt1 is constitutively expressed in proliferating cells and inactivation of this gene causes global demethylation of genomic DNA and embryonic lethality. Dnmt2 is expressed at low levels in adult tissues and its inactivation does not affect DNA methylation or maintenance of methylation. The Dnmt3 family members, Dnmt3a and Dnmt3b, are strongly expressed in ES cells but their expression is down regulated in differentiating ES cells and is low in adult somatic tissue. Dnmt1 co-purifies with the retinoblastoma (Rb) tumour suppressor gene product, E2F1, and HDAC1. Dnmt1 also cooperates with Rb to repress transcription from promoters containing E2F-binding sites suggesting a link between DNA methylation, histone deacetylase and sequence-specific DNA binding activity, as well as a growth-regulatory pathway that is disrupted in nearly all cancer cells (1-6)..
Species/HostMouse
ClonalityMonoclonal
Clone Number4G11-C7
Tested applicationsChIP, IHC, IP, WB
ReactivityFish, Human, Mouse, Zebrafish
IsotypeIgG1 Kappa
ImmunogenRaised against a partial protein corresponding to amino acids 620-950 of human DNMT1
Concentration1 mg/ml
Dilution rangeWB (1:1000), IHC (1:1000)
ConjugationATTO-390
MW180kDa
TargetDNMT1
Entrez1786
UniProt IDP26358
NCBINP_001370
StorageConjugated antibodies should be stored at 4°C
Buffer/PreservativesPBS pH 7.4, 50% glycerol, 0.09% sodium azide *Storage buffer may change when conjugated
Alternative namesDNA Mtase antibody, DNMT antibody, MCMT antibody,
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NoteFor research use only
Application notes2 µg/ml of SMC-201 was sufficient for detection of Dnmt1 in 10 µg of mouse ES cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Expiration Date12 months from date of receipt.
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