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Product Overview
Product Name DNA Polymerase zeta antibody
Catalog Number orb315685
Species/HostRabbit
ReactivityCanine, Gallus, Human, Mouse, Porcine, Rat
ConjugationUnconjugated
Tested applicationsICC, IF, IHC-P, WB
Immunogen KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human DNA Polymerase zeta. The exact sequence is proprietary.
Target REV3L. Recognizes endogenous levels of DNA Polymerase zeta protein.
Alternative Names
Product Properties
Form/Appearance Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage Store at 4°C for up to two weeks. For long term storage, aliquot and store at -20°C
Note For research use only.
Isotype IgG
ClonalityPolyclonal
Purity The antibody was purified by immunogen affinity chromatography.
Uniprot ID O60673 (Human); Q61493 (Mouse)
Entrez 5980
Product Description

Rabbit polyclonal antibody to DNA Polymerase zeta also known as hREV3 and REV3-like whose expression can be detected in the whole body ubiquitously. It is found in the nucleus. It can perform various functions like DNA-dependent DNA replication. This protein lacks an intrinsic 3'-5' exonuclease activity and thus has no proofreading function but has a catalytic subunit of the DNA polymerase zeta complex, an error-prone polymerase specialized in translation DNA synthesis (TLS).

Application Notes
Dilution Range WB: 1-500:1000, IHC-P: 1-100:200, IF/ICC: 1-100:500
Validation Images
Immunofluorescense analysis of HEK293T cells using DNA Polymerase zeta antibody
Immunofluorescense analysis of HEK293T cells using DNA Polymerase zeta antibody
Western blot analysis of K562 (Lane 1), NIH3T3 (Lane 2) whole cell lysates using DNA Polymerase zeta antibody
Western blot analysis of K562 (Lane 1), NIH3T3 (Lane 2) whole cell lysates using DNA Polymerase zeta antibody
Immunohistochemical analysis of formalin-fixed and paraffin-embedded human brain tissue using DNA Polymerase zeta antibody
Immunohistochemical analysis of formalin-fixed and paraffin-embedded human brain tissue using DNA Polymerase zeta antibody
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