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Catalog Number | orb315685 |
---|---|
Category | Antibodies |
Description | Rabbit polyclonal antibody to DNA Polymerase zeta also known as hREV3 and REV3-like whose expression can be detected in the whole body ubiquitously. It is found in the nucleus. It can perform various functions like DNA-dependent DNA replication. This protein lacks an intrinsic 3'-5' exonuclease activity and thus has no proofreading function but has a catalytic subunit of the DNA polymerase zeta complex, an error-prone polymerase specialized in translation DNA synthesis (TLS). |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IF, IH, WB |
Reactivity | Human, Mouse, Porcine, Rat |
Immunogen | KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human DNA Polymerase zeta. The exact sequence is proprietary. |
Dilution range | WB: 1-500:1000, IHC-P: 1-100:200, IF/ICC: 1-100:500 |
Form/Appearance | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
Conjugation | Unconjugated |
Target | REV3L |
Entrez | 5980, 19714 |
UniProt ID | O60673, Q61493 |
Source | Rabbit |
Storage | Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles. |
Buffer/Preservatives | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
Alternative names | anti-POLZ antibody, anti-REV3 antibody, anti-DNA p Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Immunofluorescense analysis of HEK293T cells using DNA Polymerase zeta antibody
Western blot analysis of K562 (Lane 1), NIH3T3 (Lane 2) whole cell lysates using DNA Polymerase zeta antibody
Immunohistochemical analysis of formalin-fixed and paraffin-embedded human brain tissue using DNA Polymerase zeta antibody
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