DMRT2 Antibody

• Catalog Number: orb2635205
• Category: Antibodies
• Description: The protein encoded by this gene belongs to the DMRT gene family, sharing a DM DNA-binding domain with Drosophila (dsx) and C. elegans mab3, genes involved in sex determination in these organisms. Also, this gene is located in a region of the human genome (chromosome 9p24.3) associated with gonadal dysgenesis and XY sex reversal. Hence this gene is one of the candidates for sex-determining gene(s) on chr 9. Transcriptional activator that directly regulates early activation of the myogenic determination gene MYF5 by binding in a sequence-specific manner to the early epaxial enhancer element of it. Involved in somitogenesis during embryogenesis and somite development and differentiation into sclerotome and dermomyotome. Required for the initiation and/or maintenance of proper organization of the sclerotome, dermomyotome and myotome (By similarity).
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: PCRP-DMRT2-1B11
• Tested applications: FACS, WB
• Reactivity: Human
• Isotype: Mouse IgG2b
• Immunogen: Recombinant full-length human DMRT2 protein was used as the immunogen for the DMRT2 antibody.
• Antibody Type: Primary Antibody
• Dilution range: Flow cytometry: 1-2ug/million cells,Western blot: 1-2ug/ml
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This DMRT2 antibody is available for research use only.
• UniProt ID: Q9Y5R5
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Note: For research use only
• Application notes: Optimal dilution of the DMRT2 antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

Analysis of HuProt (TM) microarray containing more than 19000 full-length human proteins using DMRT2 antibody (clone PCRP-DMRT2-1B11). These results demonstrate the foremost specificity of the PCRP-DMRT2-1B11 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

FACS staining of PFA-fixed human HeLa cells with DMRT2 antibody (blue, clone PCRP-DMRT2-1B11) and isotype control (red).

SDS-PAGE analysis of purified, BSA-free DMRT2 antibody (clone PCRP-DMRT2-1B11) as confirmation of integrity and purity.