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Catalog Number | orb1958312 |
---|---|
Category | Proteins |
Description | DLL4 Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 55.7 kDa and the accession number is Q9NR61. |
Tag | C-His |
Purity | 98.60% |
MW | 55.7 kDa (predicted); 55-70 kDa (reducing condition, due to glycosylation) |
UniProt ID | Q9NR61 |
Expression System | HEK293 Cells |
Biological Origin | Human |
Biological Activity | DLL4 Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 55.7 kDa and the accession number is Q9NR61. |
Expression Region | A DNA sequence encoding the extracellular domain (Met 1-Pro 524) of human DLL4 (NP_061947.1) pro-protein was expressed, with a polyhistidine tag at the C-terminus. Predicted N terminal: Ser 27 |
Storage | -20°C |
Note | For research use only |
Application notes | A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information. |
Expiration Date | 6 months from date of receipt. |
> 95% as determined by Tris-Bis PAGE; > 95% as determined by SEC-HPLC | |
Due to glycosylation, the protein migrates to 62-68 kDa based on Tris-Bis PAGE result. |
The purity of the protein is greater than 85% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 55.1 kDa after removal of the signal peptide. The apparent molecular mass of DLL4-His is approximately 55-70 kDa due to glycosylation. | |
Mammalian |
98.00% | |
57.55 kDa (predicted); 66.88 kDa (reducing conditions) |
98.00% | |
55.7 kDa (predicted). Due to glycosylation, the protein migrates to 62-68 kDa based on Tris-Bis PAGE result. |
> 95% by SDS-PAGE. | |
Recombinant Human DLL4 Protein is produced by Human Cell expression system. The target protein is expressed with sequence (Ser27-Pro524) of human DLL4 (Accession #Q9NR61) fused with a 10��His Tag at the C-terminus. |