DLC1 antibody

• Catalog Number: orb18877
• Category: Antibodies
• Description: Goat polyclonal antibody to DLC1
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IF, IHC
• Reactivity: Human, Mouse, Porcine
• Dilution range: ELISA: 1:64000, IHC-P: 4 μg/ml
• Conjugation: Unconjugated
• MW: 171; 50.9
• Target: DLC1 (Isoforms 1 and 3)
• Entrez: 10395
• Protein Sequence: SVAIRKRSWEEHC
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti DLC1 antibody, anti ARHGAP7 antibody, anti ST
• Note: For research use only
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.IHC: In paraffin embedded Human Adrenal Gland (Medulla) shows strong reticulate staining in the cytoplasm of medular cells. Recommended concentration, 5ug/ml.WB: Preliminary experiments gave an approx 28kDa band in Human Liver and Lung lysates as well as in different epithelial lung cell lines after 0.3 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 171 kDa according to NP_872584.1 and 54.6kDa according to NP_079043.2 (isotope 3). The 28kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported
• Expiration Date: 12 months from date of receipt.

Immunohistochemical staining of Human Adrenal Gland using DLC1 antibody