DDR1 Antibody

• Catalog Number: orb1263333
• Category: Antibodies
• Description: DDR1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, IF, IHC-P, WB
• Reactivity: Human
• Isotype: Rabbit Ig
• Immunogen: This DDR1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 299-330 amino acids from the Central region of human DDR1.
• Concentration: batch dependent
• Dilution range: For WB starting dilution is: 1:1000For IHC-P starting dilution is: 1:10~50For IF starting dilution is: 1:10~50For FACS starting dilution is: 1:10~50
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 101 kDa
• Target: DDR1
• UniProt ID: Q08345
• NCBI: Q08345
• Storage: Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: Supplied in PBS with 0.09% (W/V) sodium azide.
• Alternative names: Epithelial discoidin domain-containing receptor 1,
• Note: For research use only
• Application notes: For WB starting dilution is: 1:1000For IHC-P starting dilution is: 1:10~50For IF starting dilution is: 1:10~50For FACS starting dilution is: 1:10~50
• Expiration Date: 12 months from date of receipt.

Western blot analysis of DDR1 using rabbit polyclonal DDR1 Antibody.293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the DDR1 gene (Lane 2).

DDR1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.

Confocal immunofluorescent analysis of DDR1 Antibody with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).

Flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.