DDDDK-tag antibody

• Catalog Number: orb396143
• Category: Antibodies
• Description: Mouse monoclonal antibody to DDDDK-tag.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: M2.1
• Tested applications: ELISA, IF, WB
• Isotype: IgG
• Immunogen: Interleukin 2 genetically fused to the peptided DYKDDDDK, corresponding to the DDDDK epitope tag.
• Concentration: 1 mg/ml
• Purity: Purified
• Conjugation: Unconjugated
• Target: DDDDK-tag
• Storage: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: FLAG-tag; Equivalent to FLAG antibodies from Sigma
• Note: For research use only
• Application notes: This antibody recognises he widely used DDDDK-tag commonly used as an epitope tag in fusion proteins. The tag can be recognised at any position within a fusion protein, i.e. N-terminally, Met-N-terminally, C-terminally as well as internally. Binding of this antivbody is not sensitive to calcium. This antibody recognises both native and denatured protein.
• Expiration Date: 12 months from date of receipt.

References

Brizzard, B. L. et al. Immunoaffinity purification of FLAG epitope-tagged bacterial alkaline phosphatase using a novel monoclonal antibody and peptide elution Biotechniques, 16, 730-735 (1994)

PubMed: 8024796