DARS antibody

• Catalog Number: orb341114
• Category: Antibodies
• Description: Rabbit polyclonal antibody to DARS
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IH, WB
• Reactivity: Human, Mouse, Rat
• Immunogen: Recombinant full length protein of human DARS
• Dilution range: WB: 1:500-1:2000, IHC-P: 1:50-1:200
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: DARS
• Entrez: 1615, 226414, 116483
• UniProt ID: P15178, P14868, Q922B2
• Source: Rabbit
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: Aspartate--tRNA ligase, cytoplasmic; Aspartyl-tRNA
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of DARS expression in MCF7 (A), SW480 (B), mouse brain (C), rat liver (D) whole cell lysates. (Predicted band size: 45; 57 kD; Observed band size: 57 kD)

Immunohistochemical analysis of DARS staining in rat brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.