DAPK2 antibody

• Catalog Number: orb18331
• Category: Antibodies
• Description: Goat polyclonal antibody to DAPK2
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Reactivity: Canine, Human, Mouse, Porcine, Rat
• Dilution range: ELISA: 1:32000, WB: 1-3 μg/ml, IHC-P: 2.5 μg/ml
• Conjugation: Unconjugated
• MW: 42.9; 25.0
• Target: DAPK2
• Entrez: 23604
• Protein Sequence: KALHPRRRSSTS
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti DAPK2 antibody, anti death-associated protein
• Note: For research use only
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.IHC: Immunohistochemistry: In paraffin embedded Human Breast shows staining of the luminar side of the cell membranes of epithelial cells in the ducts. Recommended concentration, 2-4ug/ml.WB: Approx 40-45kDa bands seen in mouse brain extracts. Recommended for use at 1-3 μg/ml.
• Expiration Date: 12 months from date of receipt.

orb18331 staining (2ug/ml) of mouse brain extracts (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

orb18331 (2.5ug/ml) staining of paraffin embedded Human Breast. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Immunohistochemical staining of Human Thyroid using DAPK2 antibody