Cytokeratin 4 antibody

Catalog Number: orb98235

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CategoryPrimary antibodies
Catalog Numberorb98235
Species/HostMouse
ClonalityMonoclonal
Tested applicationsIP, WB
ReactivityCanine, Feline, Human, Zebrafish
Clone ID6B10
IsotypeIgG1
Form/AppearanceEach vial contains 100 ul 1 mg/ml purified monoclonal antibody in PBS containing 0.09% sodium azide.
ConjugationUnconjugated
UniProt IDP19013
Application notes6B10 is useful for immunocytochemistry, immunohistochemistry on frozen and tissues, immunoblotting and flow cytometry. Optimal antibody dilution should be determined by titRation; recommended range is 1:25-1:200 for flow cytometry, and for immunohistochemistry with avidin-biotinylated Horseradish peroxidase complex (ABC) as detection reagent, and 1:100-1:1000 for immunoblotting appliCations.
StorageThe antibody is shipped at ambient temperature and may be stored at +4°C. For prolonged storage prepare appropriate aliquots and store at or below -20°C. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance or the concentration of the product.
DescriptionMouse monoclonal to Cytokeratin 4
Expiration Date12 months from date of receipt.
NoteFor research use only.
Source6B10 is a Mouse monoclonal IgG1 antibody derived by fusion of SP2/0 Mouse myeloma cells with spleen cells from a BALB/c Mouse immunized with a Cytokeratin preparation extracted from Human esophagus.
  1. 4 images
    • Immunofluorescent analysis of A549 cells using Keratin, type II cytoskeletal 4 antibody
    • Immunohistochemistry of paraffin-embedded human ovarian cancer tissue using Keratin, type II cytoskeletal 4 antibody
    • Immunohistochemistry of paraffin-embedded human tonsil tissue using Keratin, type II cytoskeletal 4 antibody
    • Immunohistochemistry of paraffin-embedded human cervical cancer tissue using Keratin, type II cytoskeletal 4 antibody
    Keratin, type II cytoskeletal 4 antibody [orb241679]

    ELISA, IF, IHC

    Human

    Rabbit

    Polyclonal

    100 μg, 50 μg
  2. 3 images
    • Immunohistochemical staining of paraffin embedded human prostate carcinoma tissue using KRT4 antibody (primary antibody dilution at: 1:50-100)
    • Western blot analysis of in mouse spleen tissue lysates (35ug/lane)using KRT4 antibody (primary antibody dilution at: 1:1000)
    KRT4 antibody [orb36932]

    FC, IHC-P, WB

    Human, Mouse

    Rabbit

    Polyclonal

    Unconjugated

    400 μl, 80 μl
  3. 1 image
    • Immunohistochemical analysis of formalin fixed and paraffin embedded human liver carcinoma using pan Cytokeratin antibody
    pan Cytokeratin antibody [orb10399] Custom conjugation service available

    ELISA, FC, ICC, IF, WB, IHC

    Bovine, Canine, Equine, Human, Mouse, Porcine, Rabbit, Rat

    100 μl, 200 μl, 50 μl
  4. 1 image
    • Immunohistochemical analysis of formalin-fixed paraffin embedded rat skin tissue using Cytokeratin 4 antibody (dilution at 1:200)
    Cytokeratin 4 antibody [orb10407] Custom conjugation service available

    ELISA, FC, IF, WB, IHC

    Bovine, Canine, Equine, Human, Rabbit, Rat

    100 μl, 200 μl, 50 μl
  5. 1 image
    • Immunohistochemical staining of Human colon carcinoma using Multi Cytokeratin 4/5/6/8/10/13/18 antibody
    Cytokeratin Antibody, Multi 4/5/6/8/10/13/18 [orb248639]

    FACS, IF, IHC-P, WB

    Human, Mouse, Rat

    Mouse

    Monoclonal

    Unconjugated

    20 μg, 100 μg