Category | Primary antibodies |
---|---|
Catalog Number | orb98235 |
Species/Host | Mouse |
Clonality | Monoclonal |
Tested applications | IP, WB |
Reactivity | Canine, Feline, Human, Zebrafish |
Clone ID | 6B10 |
Isotype | IgG1 |
Form/Appearance | Each vial contains 100 ul 1 mg/ml purified monoclonal antibody in PBS containing 0.09% sodium azide. |
Conjugation | Unconjugated |
UniProt ID | P19013 |
Application notes | 6B10 is useful for immunocytochemistry, immunohistochemistry on frozen and tissues, immunoblotting and flow cytometry. Optimal antibody dilution should be determined by titRation; recommended range is 1:25-1:200 for flow cytometry, and for immunohistochemistry with avidin-biotinylated Horseradish peroxidase complex (ABC) as detection reagent, and 1:100-1:1000 for immunoblotting appliCations. |
Storage | The antibody is shipped at ambient temperature and may be stored at +4°C. For prolonged storage prepare appropriate aliquots and store at or below -20°C. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance or the concentration of the product. |
Description | Mouse monoclonal to Cytokeratin 4 |
Expiration Date | 12 months from date of receipt. |
Note | For research use only. |
Source | 6B10 is a Mouse monoclonal IgG1 antibody derived by fusion of SP2/0 Mouse myeloma cells with spleen cells from a BALB/c Mouse immunized with a Cytokeratin preparation extracted from Human esophagus. |
- Keratin, type II cytoskeletal 4 antibody [orb241679]
ELISA, IF, IHC
Human
Rabbit
Polyclonal
100 μg, 50 μg - pan Cytokeratin antibody [orb10399]
ELISA, FC, ICC, IF, WB, IHC
Bovine, Canine, Equine, Human, Mouse, Porcine, Rabbit, Rat
100 μl, 200 μl, 50 μl - Cytokeratin 4 antibody [orb10407]
ELISA, FC, IF, WB, IHC
Bovine, Canine, Equine, Human, Rabbit, Rat
100 μl, 200 μl, 50 μl - Cytokeratin Antibody, Multi 4/5/6/8/10/13/18 [orb248639]
FACS, IF, IHC-P, WB
Human, Mouse, Rat
Mouse
Monoclonal
Unconjugated
20 μg, 100 μg