Cytokeratin 13 antibody

Catalog Number: orb98274

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CategoryPrimary antibodies
Catalog Numberorb98274
Species/HostMouse
ClonalityMonoclonal
Tested applicationsIHC-Fr, IHC-P, WB
ReactivityBovine, Human, Rat
Clone IDKs13.1
IsotypeIgG1
Form/AppearanceEach vial contains 1ml of culture supernatant of monoclonal antibody containing 0.09% sodium azide.
ConjugationUnconjugated
UniProt IDP13646
Application notesKs13.1 is suitable for immunoblotting and immunohistochemistry on frozen tissues and paraffin embedded tissue after protease pretreatment. Optimal antibody dilution should be determined by titRation.
StorageThe antibody is shipped at ambient temperature and may be stored at +4°C. For prolonged storage prepare appropriate aliquots and store at or below -20°C. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance or the concentration of the product.
DescriptionMouse monoclonal to Cytokeratin 13
Expiration Date12 months from date of receipt.
NoteFor research use only.
SourceKs13.1 is a Mouse monoclonal IgG1 antibody derived by fusion of SP2/0 Mouse myeloma cells with spleen cells from a BALB/c Mouse immunized with a Cytokeratin preparation extracted from Human esophagus.
  1. 6 images
    • Immunohistochemical staining of rat brain tissue using pan Cytokeratin antibody.
    • Immunohistochemical staining of mouse lung tissue using pan Cytokeratin antibody.
    • Immunohistochemical staining of mouse lung tissue using pan Cytokeratin antibody.
    • Immunohistochemical staining of Mouse brain tissue using pan Cytokeratin antibody.
    • Immunohistochemical staining of Mouse brain tissue using pan Cytokeratin antibody.
    • Immunohistochemical staining of human colon cancer tissue using pan Cytokeratin antibody.
    pan Cytokeratin antibody [orb158093] Custom conjugation service available

    ELISA, ICC, IF, IHC

    Bovine, Equine, Human, Mouse, Rat

    100 μl, 200 μl, 50 μl
  2. 4 images
    • Immunofluorescence analysis of A431 cells using Cytokeratin 13 antibody
    • Immunohistochemical staining of Cal27 Xenograft using Cytokeratin 13 antibody
    • Immunohistochemical staining of mouse tongue using Cytokeratin 13 antibody
    • Western blot analysis of A431 whole cell lysate(Lane 1), HeLa whole cell lysate(Lane 2) using Cytokeratin 13 antibody
    Cytokeratin 13 antibody [orb556645]

    ICC, IF, IHC-P, WB

    Human, Mouse, Zebrafish

    Rabbit

    Polyclonal

    Unconjugated

    100 μl
  3. 3 images
    • Western Blot analysis of A:Hela whole cell lysate using Cytokeratin 13 antibody
    • Immunohistochemical analysis of formalin-fixed and paraffin-embedded Cal27 xenograft using Cytokeratin 13 antibody
    • Confocal immunofluorescence analysis of paraformaldehyde-fixed A431 using Cytokeratin 13 antibody
    Cytokeratin 13 antibody [orb74040] Custom conjugation service available

    IF, WB, IHC

    Human

    Rabbit

    Polyclonal

    100 μl
  4. 3 images
    • Immunohistochemical staining of paraffin embedded human breast carcinoma tissue using KRT13 antibody (primary antibody dilution at: 1:50-100)
    • Western blot analysis of in 293 cell line lysates (35ug/lane)using KRT13 antibody (primary antibody dilution at: 1:1000)
    KRT13 antibody [orb36907]

    FC, IHC-P, WB

    Human

    Rabbit

    Polyclonal

    Unconjugated

    400 μl, 80 μl
  5. 2 images
    • orb20058 (0.1ug/ml) staining of Human Lung lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
    • orb20058 (2.5ug/ml) staining of paraffin embedded Human Tonsil. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
    KRT13 antibody [orb20058]

    ELISA, WB, IHC

    Human

    Goat

    Polyclonal

    Unconjugated

    100 μg