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Product Overview
Product Name Cyclin E1 antibody
Catalog Number orb125365
Species/Host Rabbit
Reactivity Human, Mouse
Conjugation Unconjugated
Tested applications ELISA, ICC, IF, IHC-P, WB
Immunogen Synthesized non-phosphopeptide derived from human Cyclin E1 around the phosphorylation site of threonine 395 (L-L-T-P-P).
Target Cyclin E1. Cyclin E1 (Ab-395) antibody detects endogenous levels of total Cyclin E1 protein.
Product Properties
Form/Appearance PBS (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol
Concentration 1mg/ml
Storage Store at 4°C for up to two weeks. For long term storage, aliquot and store at -20°C, avoid freeze/thaw cycles.
Note For research use only.
Isotype IgG
Clonality Polyclonal
Purity The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Uniprot ID P24864
Product Description

Rabbit polyclonal antibody to Cyclin E1

Application Notes
Dilution Range WB: 1:500-1:3000, IHC-P: 1:50-1:100, IF/ICC: 1:500-1:1000, ELISA: 1:10000
Validation Images
Western blot analysis of extracts from HeLa cells using Cyclin E1 antibody
Immunohistochemical analysis of formalin-fixed and paraffin-embedded human colon carcinoma tissue using Cyclin E1 antibody
Confocal immunofluorescence analysis of HuvEc cells using Cyclin E1 antibody
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