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Product Overview
Product Name CRISP2 antibody
Catalog Number orb20468
Species/HostGoat
ReactivityCanine, Human, Porcine
ConjugationUnconjugated
Tested applicationsELISA, WB
Immunogen Peptide with sequence C-QHSDPEDRKTSTR, from the internal region of the protein sequence.
Target CRISP2
Alternative Names
Product Properties
Form/Appearance Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Storage Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles.
Note For research use only.
ClonalityPolyclonal
Purity Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
MW 35 kDa
Uniprot ID P16562
NCBI NP_003287.1
Product Description

Goat polyclonal to CRISP2

Application Notes
Dilution Range P-ELISA: 1:8000, IHC-P: 3.75μg/ml, WB: 0.5-1μg/ml
Application Notes Reported variants represent identical protein: NP_003287.1, NP_001135879.1, NP_001135889.1, NP_001135907.1, NP_001135880.1.

ELISA: Peptide ELISA: antibody detection limit dilution 1:8000.

WB: In transfected HEK293 transiently expressing Human CRISP2 (MYC and DYKDDDDK tagged), a band of approx. 35kDa was observed. No bands were observed in mock-transfected HEK293 and the same band was observed using anti-myc tag antibody. Recommended concentration, 0.5-1 μg/ml. Preliminary experiments gave an approx 55kDa band in Human Testis lysates after 1 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 27.3kDa according to NP_003287.1. The 55kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported
Validation Images
HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human CRISP2 (RC205312) with C-terminal MYC tag probed with orb20468 (1ug/ml) in Lane A and probed with anti-MYC Tag (1/1000) in lane C. Mock-transfected HEK293 probed with orb20468 (1mg/ml) in La
HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human CRISP2 (RC205312) with C-terminal MYC tag probed with orb20468 (1ug/ml) in Lane A and probed with anti-MYC Tag (1/1000) in lane C. Mock-transfected HEK293 probed with orb20468 (1mg/ml) in La
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