Collagen III antibody

• Catalog Number: orb213759
• Category: Antibodies
• Description: Rabbit polyclonal antibody to Collagen III
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IH, WB
• Reactivity: Human, Mouse, Porcine, Rabbit, Rat
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human Collagen 3 alpha 1. The exact sequence is proprietary.
• Dilution range: WB: 1-500-1-1000, IHC-P: 1-100-1-200, IF/ICC: 1-100-1-500
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: COL3A1
• Entrez: 84032, 1281, 12825
• UniProt ID: P02461, P08121, P13941
• Source: Rabbit
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: anti COL3A1 antibody, anti Collagen alpha 1(III) c
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

Guo, Fangming et al. Shenfu Administration Improves Cardiac Fibrosis in Rats With Myocardial Ischemia-Reperfusion Through Adenosine A2a Receptor Activation Hum Exp Toxicol, 41, (2022)

PubMed: 35196174

Western blot analysis of Collagen 3 alpha 1 expression in SGC7901 (A), HEK293T (B), NIH3T3 (C), H9C2 (D), PC12 (E) whole cell lysates. (Predicted band size: 138 kD; Observed band size: 139 kD)

Immunohistochemical analysis of Collagen 3 alpha 1 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Immunofluorescent analysis of Collagen 3 alpha 1 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).