COL21A1 Antibody (N-term)

• Catalog Number: orb1937708
• Category: Antibodies
• Description: Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB31769
• Tested applications: IF, IHC-P, WB
• Reactivity: Human
• Isotype: Rabbit IgG
• Antibody Type: Primary Antibody
• Dilution range: IF: 1:10~50, WB: 1:1000, IHC-P: 1:10~50
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• MW: 99369 Da
• Target: This COL21A1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 204-232 amino acids from the N-terminal region of human COL21A1.
• UniProt ID: Q96P44
• NCBI: NP_110447.2
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Collagen alpha-1(XXI) chain, COL21A1, COL1AL
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

COL21A1 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human uterus tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of COL21A1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.

COL21A1 Antibody (N-term) western blot analysis in HepG2 cell line lysates (35 ug/lane). This demonstrates the COL21A1 antibody detected the COL21A1 protein (arrow).

Confocal immunofluorescent analysis of COL21A1 Antibody (N-term) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).