CKM/Creatine Kinase MM Antibody

• Catalog Number: orb1535283
• Category: Antibodies
• Description: CKM/Creatine Kinase MM Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, IHC-P, WB
• Reactivity: Canine, Equine, Hamster, Human, Mouse
• Immunogen: Peptide with sequence NTHNKFKLNYKPEE-C, from the N-Terminus of protein sequence according to NP_001815.2.
• Concentration: 0.5 mg/ml
• Dilution range: IHC, IHC-P (5 µg/ml), Peptide-ELISA (1:128000), WB (0.01 - 0.03 µg/ml)
• Conjugation: Unconjugated
• Target: CKM / Creatine Kinase MM
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: TBS, pH 7.3, 0.02% Sodium Azide, 0.5% rAlbumin
• Alternative names: CKM, CK-MB, CKMM, Creatine kinase M-type, Creatine
• Note: For research use only
• Application notes: Further information: Peptide ELISA: antibody detection limit dilution 1:128000. Western blot: Approx 45kD band observed in Human and Mouse Skeletal Muscle and Heart lysates (calculated MW of 43.1kD according to NP_001815.2). Recommended concentration: 0.01-0.03 ug/ml.
• Expiration Date: 12 months from date of receipt.

CKM antibody (0.01 ug/ml) staining of Human (A, C) and Mouse (B, D) Skeletal Muscle (A, B) and Heart (C, D) lysates (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.