You have no items in your shopping cart.
You have no items in your shopping cart.
Catalog Number | orb385838 |
---|---|
Category | Antibodies |
Description | Chromogranin A is present in neuroendocrine cells throughout the body, including the neuroendocrine cells of the large and small intestine, adrenal medulla and pancreatic islets. It is an excellent marker for carcinoid tumors, pheochromocytomas, paragangliomas, and other neuroendocrine tumors. Co-expression of chromogranin A and neuron specific enolase (NSE) is common in neuroendocrine neoplasms. Reportedly, co-expression of certain keratins and chromogranin indicates neuroendocrine lineage. The presence of strong anti-chromogranin staining and absence of anti-keratin staining should raise the possibility of paraganglioma. The co-expression of chromogranin and NSE is typical of neuroendocrine neoplasms. Most pituitary adenomas and prolactinomas readily express chromogranin. |
Species/Host | Mouse |
Clonality | Monoclonal |
Clone Number | bGRAN9 |
Tested applications | IHC-P, WB |
Reactivity | Human |
Isotype | Mouse IgG1, kappa |
Immunogen | Recombinant human protein was used as the immunogen for the Chromogranin A antibody. |
Dilution range | Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml for 30 min at RT (1),Prediluted IHC only format: incubate for 30 min at RT (2) |
Purity | Protein G affinity chromatography |
Conjugation | Unconjugated |
Formula | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide |
Hazard Information | This Chromogranin A antibody is available for research use only. |
UniProt ID | P10645 |
Storage | Store the Chromogranin A antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide). |
Buffer/Preservatives | 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide |
Note | For research use only |
Application notes | Optimal dilution of the Chromogranin A antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of Panc-1 lysate using Chromogranin A antibody
Immunohistochemical staining of Human adrenal gland using Chromogranin A antibody
Immunohistochemical staining of human pheochromocytoma using Chromogranin A antibody
ELISA, FC, IHC, WB | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |
IHC-P, WB | |
Human, Mouse, Rat | |
Mouse | |
Monoclonal | |
Unconjugated |
Filter by Rating