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Chromogranin A Antibody

Catalog Number: orb385838

DispatchUsually dispatched within 5-10 working days
$ 764.00
Catalog Numberorb385838
CategoryAntibodies
DescriptionChromogranin A is present in neuroendocrine cells throughout the body, including the neuroendocrine cells of the large and small intestine, adrenal medulla and pancreatic islets. It is an excellent marker for carcinoid tumors, pheochromocytomas, paragangliomas, and other neuroendocrine tumors. Co-expression of chromogranin A and neuron specific enolase (NSE) is common in neuroendocrine neoplasms. Reportedly, co-expression of certain keratins and chromogranin indicates neuroendocrine lineage. The presence of strong anti-chromogranin staining and absence of anti-keratin staining should raise the possibility of paraganglioma. The co-expression of chromogranin and NSE is typical of neuroendocrine neoplasms. Most pituitary adenomas and prolactinomas readily express chromogranin.
Species/HostMouse
ClonalityMonoclonal
Clone NumberbGRAN9
Tested applicationsIHC-P, WB
ReactivityHuman
IsotypeMouse IgG1, kappa
ImmunogenRecombinant human protein was used as the immunogen for the Chromogranin A antibody.
Dilution rangeWestern blot: 1-2ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml for 30 min at RT (1),Prediluted IHC only format: incubate for 30 min at RT (2)
PurityProtein G affinity chromatography
ConjugationUnconjugated
Formula0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Hazard InformationThis Chromogranin A antibody is available for research use only.
UniProt IDP10645
StorageStore the Chromogranin A antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
Buffer/Preservatives0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
NoteFor research use only
Application notesOptimal dilution of the Chromogranin A antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Expiration Date12 months from date of receipt.
Chromogranin A Antibody

Western blot analysis of Panc-1 lysate using Chromogranin A antibody

Chromogranin A Antibody

Immunohistochemical staining of Human adrenal gland using Chromogranin A antibody

Chromogranin A Antibody

Immunohistochemical staining of human pheochromocytoma using Chromogranin A antibody

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