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Catalog Number | orb234297 |
---|---|
Category | Antibodies |
Description | CD86 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IHC, WB |
Reactivity | Human, Mouse |
Isotype | Rabbit IgG |
Immunogen | E.coli-derived human CD86 recombinant protein (Position: A24-F329). Human CD86 shares 50% amino acid (aa) sequence identity with mouse CD86. |
Concentration | Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
Dilution range | Western blot, 0.1-0.5μg/ml Immunohistochemistry(Paraffin-embedded Section), 2-5μg/ml |
Form/Appearance | Lyophilized |
Conjugation | Unconjugated |
MW | 37682 MW |
UniProt ID | P42081 |
Storage | Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles. |
Alternative names | T-lymphocyte activation antigen CD86;Activation B7 Read more... |
Note | For research use only |
Application notes | WB: The detection limit for CD86 is approximately 0.25ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml. |
Expiration Date | 12 months from date of receipt. |
WB analysis of CD86 using anti-CD86 antibody.Lane 1:human Raji cell;2:human Daudi cell.
WB analysis of CD86 using anti-CD86 antibody.Lane 1:mouse spleen tissue;2:mouse thymus tissue;3:mouse J774A.1 cell;4:mouse Ana-1 cell.
IHC analysis of CD86 using anti-CD86 antibody. CD86 was detected in a paraffin-embedded section of human tonsil tissue.
ELISA, IF, IHC-Fr, IHC-P, WB | |
Bovine, Canine, Porcine, Sheep | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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