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Catalog Number | orb215924 |
---|---|
Category | Antibodies |
Description | CD82 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IHC, WB |
Reactivity | Human |
Isotype | Rabbit IgG |
Immunogen | E.coli-derived human CD82 recombinant protein (Position: A98-Y267). Human CD82 shares 69% and 67% amino acid (aa) sequences identity with mouse and rat CD82, respectively. |
Concentration | Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
Dilution range | Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human, By HeatWestern blot, 0.1-0.5μg/ml, Human |
Form/Appearance | Lyophilized |
Conjugation | Unconjugated |
MW | 29626 MW |
UniProt ID | P27701 |
Storage | Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles. |
Alternative names | CD82 antigen;C33 antigen;IA4;Inducible membrane pr Read more... |
Note | For research use only |
Application notes | WB: The detection limit for CD82 is approximately 0.25ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml. |
Expiration Date | 12 months from date of receipt. |
WB analysis of CD82 using anti-CD82 antibody.Lane 1:human Jurkat cell; 2:human Raji cell; 3:human U-87MG cell.
IHC analysis of CD82 using anti-CD82 antibody. CD82 was detected in a paraffin-embedded section of human tonsil tissue.
IHC analysis of CD82 using anti-CD82 antibody. CD82 was detected in a paraffin-embedded section of human tonsil tissue.
ICC, IF, IHC, WB | |
Human, Mouse, Rat | |
Polyclonal | |
Unconjugated |
ELISA, IF, IHC-P, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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