CD70 Antibody [TAN1-6], Rabbit IgG

• Catalog Number: orb1671770
• Category: Antibodies
• Description: CD70 Antibody [TAN1-6], Rabbit IgG
• Clonality: Recombinant
• Clone Number: TAN1-6
• Tested applications: Blocking, FC, IF, WB
• Reactivity: Mouse
• Isotype: IgG kappa
• Immunogen: This antibody was raised by immunising LOU rats with soluble recombinant CD70:Fc fusion protein
• Antibody Type: Primary Antibody
• Concentration: batch dependent
• Conjugation: Unconjugated
• Target: Cd70
• UniProt ID: O55237
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: TNFSF7, CD27 ligand, CD27-L, Cd27l, CD27LG, CD70 a
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Immunofluorescence staining of fixed RAW264.7 cells with anti-CD70 antibody TAN1-6. Immunofluorescence analysis of paraformaldehyde fixed RAW264.7 cells on Shi-fix™ coverslips stained with the chimeric r version of TAN1-6 (orb1671770) at 10 ug/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 ug/ml)- showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom orb1671770- DAPI- merged channels and an isotype control. The isotype control was an unknown specificity antibody (.1) followed by staining with Alexa Fluor® 488 secondary antibody.

Flow cytometry using the Anti-CD70 antibody TAN1-6. Paraformaldehyde fixed mouse splenocytes permewith 0.5% Triton were stained with anti-unknown specificity antibody (3.0; isotype control - black line) or the r version of TAN1-6 (orb1671770 - blue line) at a dilution of 1:100 for 1h at RT. After washing- the bound antibody was detected using a goat anti-r AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.