CD47 antibody

• Catalog Number: orb4838
• Category: Antibodies
• Description: CD47 antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, WB
• Predicted Reactivity: Rabbit
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human CD47/MER6 (241-323/323 aa)
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, Flow-Cyt=2μg/Test, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, ELISA=1:5000-10000
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 33 kDa
• Target: CD47
• UniProt ID: Q08722
• Storage: Shipped at 4°C. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
• Buffer/Preservatives: 0.01M TBS(pH7.4) with 1% rAlbumin, 0.03% Proclin300 and 50% Glycerol.
• Alternative names: Antigenic surface determinant protein OA3; CD 47;
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Paraffin-embedded rat tongue tissue fixed with 4% paraformaldehyde. Antigen retrieval by boiling with citrate buffer. Blocking buffer is goat serum (37 degrees for 20 min.). CD47 antibody at 1:200 dilution with overnight incubation at 4 degrees

Paraffin-embedded rat colitis tissue fixed with 4% paraformaldehyde. Antigen retrieval by boiling with citrate buffer. Blocking buffer is goat serum (37 degrees for 20 min.). CD47 antibody at 1:200 dilution with overnight incubation at 4 degrees