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CD4 antibody

Catalog Number: orb248919

DispatchUsually dispatched within 5-10 working days
$ 267.00
Catalog Numberorb248919
CategoryAntibodies
DescriptionMouse monoclonal antibody to CD4
Species/HostMouse
ClonalityMonoclonal
Clone NumberRiv7
Tested applicationsFC, IF
ReactivityHuman
ImmunogenNative purified human CD4.
Form/AppearanceMouse IgG2a kappa. Liquid in PBS, pH 7.3, 0.2% rAlbumin, and 0.02% sodium azide.
ConjugationUnconjugated
TargetCD4
Entrez920
UniProt IDP01730
SourceMouse
StorageShipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
Buffer/PreservativesMouse IgG2a kappa. Liquid in PBS, pH 7.3, 0.2% rAlbumin, and 0.02% sodium azide.
Alternative namesAnti-CD 4 antibody, Anti-CD4 (L3T4) antibody, Anti
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NoteFor research use only
Application notes1. Take 100 μl peripheral blood anticoagulated by EDTA and add to the bottom of 5 ml tube. 2. Add appropriate amount of antibody to the bottom of flow tube mixing with the whole blood; incubate for 30 minutes at room temperature. 3. Add 2 ml RBC lysis buffer; incubate for 10 minutes after mixing; dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes; discard the supernatant. 5. Add 2 ml PBS wash buffer to resuspend the cells; then 1000 rpm centrifugation for 5 minutes; discard the supernatant. 6. Add appropriate amount of fluorescent-labeled anti-mouse IgGs and incubate for 20 minutes away from light at room temperature. 7. Add 2 ml PBS wash buffer to resuspend the cells; then 1000 rpm centrifugation for 5 minutes; discard the supernatant. 8. Add 0.5 ml PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).
Expiration Date12 months from date of receipt.
CD4 antibody

Flow cytometric analysis of human peripheral blood lymphocytes using CD4 antibody

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