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CD4 antibody

Catalog Number: orb222770

DispatchUsually dispatched within 5-10 working days
$ 219.00
Catalog Numberorb222770
CategoryAntibodies
DescriptionRat monoclonal antibody to CD4
Species/HostRat
ClonalityMonoclonal
Clone NumberGK1.5
Tested applicationsFA, FC, ICC, IHC-Fr, IP
ReactivityMouse
IsotypeRat IgG2b
ImmunogenMouse CTL clone V4 cells
Concentration1 mg/ml
Dilution rangeFunctional application: Isolation and depletion of CD4+ T cells, blocking of ligand binding to CD4.
Immunocytochemistry: Recommended dilution: 1-4 μg/ml.
Immunoprecipitation: Recommended dilution: 1-2 μg / 100-500 μg of protein in 1 ml lysate.
Flow cytometry: Recommended dilution: 1 μg/million cells.
PurityPurified by protein-G affinity chromatography.
ConjugationUnconjugated
TargetCD4
Entrez12504
UniProt IDP06332
StorageStore at 2-8°C. Do not freeze.
Buffer/PreservativesPhosphate buffered saline (PBS), pH 7.4
Alternative namesAnti-CD 4 antibody, Anti-CD4 (L3T4) antibody, Anti
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NoteFor research use only
Application notesLow Endotoxin, Azide free format. Product does not contain preservatives and is intended primarily for functional applications. Preservative containing buffers are available for IHC applications
Expiration Date12 months from date of receipt.
CD4 antibody

Separation of murine CD4 positive cells (red-filled) from murine CD4 negative cells (black-dashed) in flow cytometry analysis (surface staining) of murine splenocyte suspension stained using anti-mouse CD4 (GK1.5) purified antibody (low endotoxin, concentration in sample 4 μg/ml) DAR APC.

CD4 antibody

Flow cytometry surface staining pattern of murine splenocyte suspension stained using anti-mouse CD4 (GK1.5) purified antibody (low endotoxin, concentration in sample 4 μg/ml) DAR APC.

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