CD4 antibody

• Catalog Number: orb222769
• Category: Antibodies
• Description: Rat monoclonal antibody to CD4
• Clonality: Monoclonal
• Clone Number: GK1.5
• Tested applications: FC, ICC, IHC-Fr, IP
• Reactivity: Mouse
• Isotype: Rat IgG2b
• Immunogen: Mouse CTL clone V4 cells
• Concentration: 1 mg/ml
• Dilution range: Immunocytochemistry: Recommended dilution: 1-4 μg/ml. Immunoprecipitation: Recommended dilution: 1-2 μg / 100-500 μg of protein in 1 ml lysate. Flow cytometry: Recommended dilution: 1 μg/million cells.
• Purity: Purified by protein-G affinity chromatography.
• Conjugation: Unconjugated
• Target: CD4
• Entrez: 12504
• UniProt ID: P06332
• Storage: Store at 2-8°C. Do not freeze.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Alternative names: Anti-CD 4 antibody, Anti-CD4 (L3T4) antibody, Anti
• Note: For research use only
• Application notes: Immunocytochemistry: Recommended dilution: 1-4 μg/ml. Immunoprecipitation: Recommended dilution: 1-2 μg / 100-500 μg of protein in 1 ml lysate. Flow cytometry: Recommended dilution: 1 μg/million cells.
• Expiration Date: 12 months from date of receipt.

Separation of murine CD4 positive cells (red-filled) from murine CD4 negative cells (black-dashed) in flow cytometry analysis (surface staining) of murine splenocyte suspension stained using anti-mouse CD4 (GK1.5) purified antibody (concentration in sample 4 μg/ml) DAR APC.

Flow cytometry surface staining pattern of murine splenocyte suspension stained using anti-mouse CD4 (GK1.5) purified antibody (concentration in sample 4 μg/ml) DAR APC.