CD35 Antibody

• Catalog Number: orb639598
• Category: Antibodies
• Description: Recognizes a protein of 210-220kDa, which is identified as the complement receptor 1 (CR1)/CD35. This MAb does not block CR1 activity. It is highly specific to CR1 and shows no cross-reaction with CR2. The primary function of CR1 is to serve as the cellular receptor for C3b and C4b, the most important components of the complement system leading to clearance of foreign macromolecules. The Knops blood group system is a system of antigens located on this protein.Follicular dendritic cells (FDC) are restricted to the B-cell regions of secondary lymphoid follicles. They are CD21+/CD35+/CD1a-. This MAb labels follicular dendritic cells and follicular dendritic cell sarcoma.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: To5
• Tested applications: FACS, IF, IHC-P
• Reactivity: Human
• Isotype: Mouse IgG1, kappa
• Immunogen: Follicular dendritic cells (FDC) from the tonsil were used as the immunogen for the CD35 antibody.
• Dilution range: Flow cytometry: 1-2ug/million cells,,Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This CD35 antibody is available for research use only.
• UniProt ID: P17927
• Storage: Store the CD35 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.