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CD282 antibody

Catalog Number: orb248906

DispatchUsually dispatched within 5-10 working days
$ 413.00
Catalog Numberorb248906
CategoryAntibodies
DescriptionMouse monoclonal antibody to CD282
Species/HostMouse
ClonalityMonoclonal
Clone NumberT2.5
Tested applicationsFC, IF
ReactivityHuman
ImmunogenNative purified human CD282.
Form/AppearanceMouse IgG2b. Liquid in PBS, pH 7.3, and 0.02% sodium azide.
ConjugationUnconjugated
TargetTLR2
Entrez7097
UniProt IDO60603
SourceMouse
StorageShipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
Buffer/PreservativesMouse IgG2b. Liquid in PBS, pH 7.3, and 0.02% sodium azide.
Alternative namesanti TLR2 antibody, anti TIL4 antibody, anti Toll-
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NoteFor research use only
Application notes1. Take 100 μl peripheral blood anticoagulated by EDTA and add to the bottom of 5 ml tube. 2. Add appropriate amount of antibody to the bottom of flow tube mixing with the whole blood; incubate for 30 minutes at room temperature. 3. Add 2 ml RBC lysis buffer; incubate for 10 minutes after mixing; dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes; discard the supernatant. 5. Add 2 ml PBS wash buffer to resuspend the cells; then 1000 rpm centrifugation for 5 minutes; discard the supernatant. 6. Add appropriate amount of fluorescent-labeled anti-mouse IgGs and incubate for 20 minutes away from light at room temperature. 7. Add 2 ml PBS wash buffer to resuspend the cells; then 1000 rpm centrifugation for 5 minutes; discard the supernatant. 8. Add 0.5 ml PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).
Expiration Date12 months from date of receipt.
CD282 antibody

Flow cytometric analysis of human peripheral blood monocytes using CD282 antibody

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