CD28 antibody

• Catalog Number: orb44737
• Category: Antibodies
• Description: Mouse Monoclonal to CD28.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: JJ319
• Tested applications: FC, IP
• Reactivity: Rat
• Isotype: Mouse IgG1 kappa
• Immunogen: Mouse A20J B lymphoma cells transfected with rat CD28
• Concentration: 1 mg/ml
• Dilution range: Flow cytometry: Recommended dilution: 3-12 μg/ml.
• Purity: Purified by protein-A affinity chromatography.
• Conjugation: Unconjugated
• Target: CD28
• Entrez: 25660
• UniProt ID: P31042
• Storage: Store at 2-8°C. Do not freeze.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Alternative names: Anti-CD28 (rat) antibody
• Note: For research use only
• Application notes: Flow cytometry: Recommended dilution: 3-12 μg/ml.
• Expiration Date: 12 months from date of receipt.

Separation of rat CD28 positive splenocytes (red-filled) from CD28 negative splenocytes (black-dashed) in flow cytometry analysis (surface staining) of rat splenocytes suspension stained using anti-rat CD28 (JJ319) purified antibody (concentration in sample 4 μg/ml) GAM APC.

Flow cytometry surface staining pattern of rat splenocytes suspension stained using anti-rat CD28 (JJ319) purified antibody (concentration in sample 4 μg/ml) GAM APC.