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Catalog Number | orb154382 |
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Category | Antibodies |
Description | Mouse monoclonal antibody to CD271 which is low affinity receptor which can bind to NGF, BDNF, NT-3, and NT-4. It can mediate cell survival as well as cell death of neural cells. CD271 is useful for the detection of all cells expressing p75NTR on their surface, such as motor neurons cholinergic basal forebrain neurons, activated Schwann cells, olfactory ensheathing glial cells. |
Species/Host | Mouse |
Clonality | Monoclonal |
Clone Number | NGFR5 |
Tested applications | CyTOF, FC, ICC, IHC-P, IP, WB |
Reactivity | Feline, Ferret, Human, Primate, Rabbit |
Isotype | Mouse IgG1 |
Immunogen | Purified CD271 protein isolated from human melanoma cell line A875 |
Concentration | 1 mg/ml |
Dilution range | Immunohistochemistry (paraffin sections): Recommended dilution: 1-2 μg/ml; Positive tissue: melanoma, heat-mediated antigen retrieval. Flow cytometry: Extracellular and intracellular staining; recommended dilution: 1-4 µg/ml. |
Purity | Purified by protein-A affinity chromatography. |
Conjugation | Unconjugated |
Target | CD271 |
Entrez | 4804 |
UniProt ID | P08138 |
Storage | Store at 2-8°C. Do not freeze. |
Buffer/Preservatives | Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
Alternative names | Anti-TNFRSF16 antibody, anti-NGFR antibody, anti-p Read more... |
Note | For research use only |
Application notes | Immunohistochemistry (paraffin sections): Recommended dilution: 1-2 μg/ml; Positive tissue: melanoma, heat-mediated antigen retrieval. Flow cytometry: Extracellular and intracellular staining; recommended dilution: 1-4 µg/ml. |
Expiration Date | 12 months from date of receipt. |
Flow cytometric analysis of brain tumor cells using CD271 antibody
ELISA, FC, ICC, IF, WB | |
Human, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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