CD261 antibody

• Catalog Number: orb154420
• Category: Antibodies
• Description: Mouse monoclonal antibody to CD261 which is a receptor for the cytotoxic ligand TNFSF10/TRAIL. The adapter molecule FADD recruits caspase-8 to activated CD261 which is expressed in most human tissues including spleen, peripheral blood leucocytes, thymus and in a variety oFT tumour-derived cell lines. It plays a role in inducing cell death. The binding oFT TRAIL to DR4 triggers the activation oFT pro-caspases 8 and 10, leading to apoptosis.
• Clonality: Monoclonal
• Clone Number: DR-4-02
• Tested applications: FA, FC, ICC, IP
• Reactivity: Human
• Isotype: Mouse IgG1
• Immunogen: Fusion protein containing the extracellular part of TRAIL-R1 and the constant part of the heavy chain of the human IgG1.
• Concentration: 1 mg/ml
• Dilution range: Functional application: Soluble antibody DR-4-02 blocks apoptosis triggered by a ligand (TRAIL). Plastic-immobilized (cross-linked) DR-4-02 antibody induces apoptosis in sensitive cells. Recommended dilution of antibody: 2-3 μg/ml in cultivation medium, final concentration of TRAIL: 20-200 ng/ml; it is recommended to add the antibody 15 min before addition of TRAIL. Flow cytometry: Recommended dilution: 2-4 μg/ml.
• Purity: Purified by protein-A affinity chromatography.
• Conjugation: Unconjugated
• Target: CD261
• Entrez: 8797
• UniProt ID: O00220
• Storage: Store at 2-8°C. Do not freeze.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4
• Alternative names: Anti-DR4 antibody, anti-APO2 antibody, anti-TNFRSF
• Note: For research use only
• Application notes: Low Endotoxin, Azide free format. Product does not contain preservatives and is intended primarily for functional applications. Preservative containing buffers are available for IHC applications
• Expiration Date: 12 months from date of receipt.

Flow cytometry analysis of TRAIL-R1 expression on the surface of hematopoietic cell lines. Cells were stained with purified anti-TRAIL-R1 purified antibodies (low endotoxin) followed by Goat anti-mouse IgG PE.