CD253 antibody (PE )

• Catalog Number: orb154497
• Category: Antibodies
• Description: Mouse monoclonal antibody conjugated to PE which recognizes CD253 that is also known as TNFSF10 is a cytokine that is a member of the tumor necrosis factor (TNF) ligand family. It highly expressed in spleen, lung and prostate. CD253 is commonly used as cell markers in immunophynotyping and also induces apoptosis
• Clonality: Monoclonal
• Clone Number: 2E5
• Tested applications: FC
• Reactivity: Human
• Isotype: Mouse IgG1
• Immunogen: Recombinant soluble fragment (aa 95-281) of human TRAIL.
• Concentration: 0.1 mg/ml
• Dilution range: Flow cytometry: Recommended dilution: 1-5 μg/ml.
• Purity: Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
• Conjugation: PE
• Target: CD253
• Entrez: 8743
• UniProt ID: P50591
• Storage: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
• Buffer/Preservatives: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Alternative names: Anti-TRAIL antibody, anti-Apo-2 ligand antibody, a
• Note: For research use only
• Application notes: Flow cytometry: Recommended dilution: 1-5 μg/ml.
• Expiration Date: 12 months from date of receipt.

Anti-Hu CD253 PE antibody (clone 2E5) specificity verification by flow cytometry Colorectal cancer cell line HCT-116 with the eliminated expression of pro-apoptotic proteins BAK and BAX (prepared using CRISPR Cas9 gene editing approach) was used as host for CD253 (TRAIL) transfection. BAK/BAX double knockout cells are resistant to TRAIL-induced apoptosis. Anti-Hu CD253 PE (concentration in sample 5 μg/ml, red-filled histogram) binds specifically to surface CD253/TRAIL in transfected cells (upper panel), but not to the parent HCT-166 BAK/BAK double KO (lower panel). Level of non-specific binding was assessed using Mouse IgG1 isotype control PE (MOPC-21) under same conditions (concentration in sample 5 μg/ml, black-dashed histogram).