CD253 antibody

• Catalog Number: orb222728
• Category: Antibodies
• Description: Mouse monoclonal antibody to CD253
• Clonality: Monoclonal
• Clone Number: 2E5
• Tested applications: FA, FC
• Reactivity: Human
• Isotype: Mouse IgG1
• Immunogen: Recombinant soluble fragment (aa 95-281) of human TRAIL.
• Concentration: 1 mg/ml
• Dilution range: Functional application: The antibody 2E5 has high neutralizing activity for human TRAIL in biological assays. Flow cytometry: Recommended dilution: 1-10 μg/ml.
• Purity: Purified by protein-A affinity chromatography.
• Conjugation: Unconjugated
• Target: CD253
• Entrez: 8743
• UniProt ID: P50591
• Storage: Store at 2-8°C. Do not freeze.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4
• Alternative names: TRAIL
• Note: For research use only
• Application notes: Low Endotoxin, Azide free format. Product does not contain preservatives and is intended primarily for functional applications. Preservative containing buffers are available for IHC applications
• Expiration Date: 12 months from date of receipt.

Functional applicvation example: Apoptosis induced in JURKAT human T cell leukemia cell line by soluble recombinant human TRAIL is completely blocked by anti-human TRAIL (2E5). The neutralizing activity of the antibody 2E5 has been confirmed with various sources of soluble recombinant human TRAIL. A - medium B - recombinant TRAIL C - recombinant TRAIL + anti-human TRAIL (2E5; 0.06 μg/ml) D - recombinant TRAIL + anti-human TRAIL (2E5; 0.24 μg/ml) E - recombinant TRAIL + Isotype mouse IgG1 control