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Catalog Number | orb222728 |
---|---|
Category | Antibodies |
Description | Mouse monoclonal antibody to CD253 |
Clonality | Monoclonal |
Clone Number | 2E5 |
Tested applications | FA, FC |
Reactivity | Human |
Isotype | Mouse IgG1 |
Immunogen | Recombinant soluble fragment (aa 95-281) of human TRAIL. |
Concentration | 1 mg/ml |
Dilution range | Functional application: The antibody 2E5 has high neutralizing activity for human TRAIL in biological assays. Flow cytometry: Recommended dilution: 1-10 μg/ml. |
Purity | Purified by protein-A affinity chromatography. |
Conjugation | Unconjugated |
Target | CD253 |
Entrez | 8743 |
UniProt ID | P50591 |
Storage | Store at 2-8°C. Do not freeze. |
Buffer/Preservatives | Phosphate buffered saline (PBS), pH 7.4 |
Alternative names | TRAIL Read more... |
Note | For research use only |
Application notes | Low Endotoxin, Azide free format. Product does not contain preservatives and is intended primarily for functional applications. Preservative containing buffers are available for IHC applications |
Expiration Date | 12 months from date of receipt. |
Functional applicvation example: Apoptosis induced in JURKAT human T cell leukemia cell line by soluble recombinant human TRAIL is completely blocked by anti-human TRAIL (2E5). The neutralizing activity of the antibody 2E5 has been confirmed with various sources of soluble recombinant human TRAIL. A - medium B - recombinant TRAIL C - recombinant TRAIL + anti-human TRAIL (2E5; 0.06 μg/ml) D - recombinant TRAIL + anti-human TRAIL (2E5; 0.24 μg/ml) E - recombinant TRAIL + Isotype mouse IgG1 control
ELISA, IF, IHC-P, WB | |
Mouse | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |
IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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