CD222 antibody (biotin)

• Catalog Number: orb402816
• Category: Antibodies
• Description: Mouse monoclonal antibody against CD222 conjugated to biotin
• Clonality: Monoclonal
• Clone Number: MEM-238
• Tested applications: FC, IP, WB
• Reactivity: Human, Primate
• Isotype: Mouse IgG1
• Immunogen: Recombinant Vaccinia virus encoding CD222.
• Concentration: 1 mg/ml
• Dilution range: Flow cytometry: Extracellular and intracellular staining; recommended dilution: 2-6 μg/ml. Western blotting: Recommended dilution: 1 µg/ml; non-reducing conditions.
• Purity: Purified antibody is conjugated with biotin LC-NHS ester under optimum conditions and unconjugated antibody and free biotin are removed by size-exclusion chromatography.
• Conjugation: Biotin
• Target: CD222
• Entrez: 3482
• UniProt ID: P11717
• Storage: Store at 2-8°C. Do not freeze.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Alternative names: Anti-IGF2R antibody, anti-MPR1 antibody, anti-CIMP
• Note: For research use only
• Application notes: Flow cytometry: Extracellular and intracellular staining; recommended dilution: 2-6 μg/ml.Western blotting: Recommended dilution: 1 µg/ml; non-reducing conditions.
• Expiration Date: 12 months from date of receipt.

Flow cytometry analysis (surface staining) of human peripheral blood with anti-CD222 (MEM-238) biotin, streptavidin-APC.

Anti-Hu CD222 Biotin (clone MEM-238) works in WB application under non-reducing conditions. Western blotting analysis was performed on whole cell extracts (RIPA lysis buffer) of Raji, Jurkat, HeLa, and K562 cell lines, mixed and heated (100°C, 5 min) with non-reducing SDS-loading buffer. Samples were resolved using 7% Tris-glycine SDS gel electrophoresis. Nitrocellulose membrane blot was probed with biotinylated mouse IgG1 monoclonal antibody MEM-238 (1 µg/ml), followed by IRDye 800CW Streptavidin (green). Multiplex fluorescent Western blot detection was performed. CD222 molecules were detected at ~250 kDa in all analysed cell lines.