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CD206 antibody

Catalog Number: orb308746

DispatchUsually dispatched within 5-10 working days
$ 275.00
Catalog Numberorb308746
CategoryAntibodies
DescriptionMouse monoclonal antibody to CD206
Species/HostMouse
ClonalityMonoclonal
Clone Number15-2
Tested applicationsFA, FC, ICC, IHC-Fr, IP, WB
ReactivityHuman
IsotypeMouse IgG1 kappa
ImmunogenPurified human mannose receptor
Concentration1 mg/ml
Dilution rangeFunctional application: Blocking. Flow cytometry: Recommended dilution: 1-4 µg/ml. Western blotting: Recommended dilution: 1-2 µg/ml.
PurityPurified by protein-A affinity chromatography.
ConjugationUnconjugated
TargetCD206
Entrez4360
UniProt IDP22897
StorageStore at 2-8°C. Do not freeze.
Buffer/PreservativesPhosphate buffered saline (PBS), pH 7.4
Alternative namesAnti-MMR antibody, anti-MRC1 antibody, anti-CLEC13
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NoteFor research use only
Application notesLow Endotoxin, Azide free format. Product does not contain preservatives and is intended primarily for functional applications. Preservative containing buffers are available for IHC applications
Expiration Date12 months from date of receipt.
CD206 antibody

Separation of human CD206 positive dendritic cells differentiated upon monocyte stimulation (GM-CSF + IL-4) (red-filled) from non-stimulated lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human stimulated (GM-CSF + IL-4) peripheral blood mononuclear cells stained using anti-human CD206 (15-2) purified antibody (low endotoxin, concentration in sample 9 μg/ml), GAM PE.

CD206 antibody

Flow cytometry surface staining pattern of human stimulated (GM-CSF + IL-4) peripheral blood mononuclear cells stained using anti-human CD206 (15-2) purified antibody (low endotoxin, concentration in sample 9 μg/ml), GAM PE.

CD206 antibody

Western blotting analysis of human CD206 using mouse monoclonal antibody 15-2 on lysates of differentiated monocytes (two batches), and on THP-1 and Raji cells (negative controls) under reducing and non-reducing conditions. Nitrocellulose membrane was probed with 2 µg/ml of mouse monoclonal antibody followed by IRDye800-conjugated anti-mouse secondary antibody. CD206 was detected around 170 kDa.

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    ELISA,  IHC-P,  WB

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