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Item 1 of 4
CD1E Antibody (Center)
Catalog Number: orb1927630
| Catalog Number | orb1927630 |
|---|---|
| Category | Antibodies |
| Description | Affinity Purified Rabbit Polyclonal Antibody (Pab) |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Clone Number | RB26783 |
| Tested applications | FC, IF, IHC-P, WB |
| Reactivity | Human |
| Isotype | Rabbit IgG |
| Antibody Type | Primary Antibody |
| Dilution range | IF: 1:10~50, WB: 1:1000, IHC-P: 1:50~100, FC: 1:10~50 |
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Conjugation | Unconjugated |
| MW | 43626 Da |
| Target | This CD1E antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 184-212 amino acids from the Central region of human CD1E. |
| UniProt ID | P15812 |
| NCBI | NP_001172037.1, NP_001036050.1, NP_112155.2, NP_001172036.1, NP_001172039.1, NP_001172042.1, NP_001172044.1, NP_001172041.1, NP_001036051.1, NP_001172043.1, NP_001036048.1, NP_001036049.1, NP_001036052.1 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
| Alternative names | T-cell surface glycoprotein CD1e, membrane-associa Read more... |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
CD1E Antibody (Center) western blot analysis in MDA-MB435 cell line lysates (35 ug/lane).This demonstrates the CD1E antibody detected the CD1E protein (arrow).
Confocal immunofluorescent analysis of CD1E Antibody (Center) with MDA-MB435 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).
CD1E Antibody (Center) flow cytometric analysis of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
CD1E Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human spleen followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the CD1E Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
