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Catalog Number | orb154416 |
---|---|
Category | Antibodies |
Description | Mouse monoclonal antibody to CD18 which belongs to the integrin beta chain family. Defects in ITGB2 are the cause of leukocyte adhesion deficiency type 1 (LAD1). CD18 is involved in cellular adhesion and the immune response. CD18 and the a subunits are expressed broadly by all leukocytes with NK and T cells showing higher density of surface expression. |
Species/Host | Mouse |
Clonality | Monoclonal |
Clone Number | MEM-48 |
Tested applications | FA, FC, IHC-Fr, IHC-P, IP, WB |
Reactivity | Human |
Isotype | Mouse IgG1 |
Immunogen | Leukocytes of a patient suffering from a LGL-type leukemia. |
Concentration | 1 mg/ml |
Dilution range | Functional application: The antibody MEM-48 induces high-affinity conformation of LFA-1 complex. Western blotting: Recommended dilution: 2 μg/ml; positive control: Kg-1a human leukemia cell lysate, non-reducing conditions. Flow cytometry: Recommended dilution: 2 μg/ml. Immunohistochemistry (paraffin sections): Recommended dilution: 10 μg/ml; positive tissue: spleen, microglia. |
Purity | Purified by sequential steps of physicochemical fractionation (differential precipitation and solid-phase chromatography methods). |
Conjugation | Unconjugated |
Target | CD18 |
Entrez | 3689 |
UniProt ID | P05107 |
Storage | Store at 2-8°C. Do not freeze. |
Buffer/Preservatives | Phosphate buffered saline (PBS), pH 7.4 |
Alternative names | Anti-Integrin beta2 antibody, anti-Complement rece Read more... |
Note | For research use only |
Application notes | Low Endotoxin, Azide free format. Product does not contain preservatives and is intended primarily for functional applications. Preservative containing buffers are available for IHC applications |
Expiration Date | 12 months from date of receipt. |
Separation of human leukocytes (red-filled) from CD18 negative blood debris (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD18 (MEM-48) purified antibody (concentration in sample 1 μg/ml, GAM APC).
Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD18 (MEM-48) purified antibody (concentration in sample 1 μg/ml, GAM APC).
Immunohistochemistry staining of human spleen (paraffin sections) using anti-CD18 (MEM-48).
ELISA, FC, IF, IHC-Fr, IHC-P, WB | |
Porcine | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ICC, IF, IHC, WB | |
Human, Mouse, Rat | |
Polyclonal | |
Unconjugated |
ELISA, FC, ICC, IF, IHC-Fr, IHC-P | |
Bovine, Canine, Equine, Gallus, Guinea pig, Mouse, Porcine, Rabbit, Rat, Sheep | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |
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