CD163 Antibody

• Catalog Number: orb699535
• Category: Antibodies
• Description: This MAb recognizes a protein of 140kDa, identified as CD163. It has been identified as an acute phase-regulated transmembrane protein whose function is to mediate the endocytosis of haptoglobin-hemoglobin complexes. This receptor is expressed on the surface of monocytes with low expression and on tissue macrophages, histiocytes with high expression. Staining with anti-CD163 has been helpful to distinguish synovial macrophages from synovial intimal fibroblasts in rheumatoid arthritis, where its specificity for macrophages was found to be superior to that of anti-CD68. Increased levels of CD163 were also detected in patients with microbial infections and myelomonocytic leukemias. Anti-CD163 is of considerable value for selective identification of monocytes and macrophages at a certain stage of differentiation and is suitable for diagnosing myelomonocytic or monocytic leukaemia and neoplasms of true histiocytic origin. CD163 is positive in skin (histiocytes), gut, Kupffer cells, a few alveolar macrophages, macrophages in the placenta, and in macrophages in inflamed tissues including tumor tissue.
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: Mouse IgG1, kappa
• Conjugation: Unconjugated
• Reactivity: Human
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide
• Purity: Protein G affinity chromatography
• Immunogen: A portion of amino acids 43-196 from the human protein was used as the immunogen for the CD163 antibody.
• UniProt ID: Q86VB7
• Tested applications: IHC-P
• Dilution range: Immunohistochemistry (FFPE): 1-2ug/ml for 30 minutes at RT
• Application notes: Optimal dilution of the CD163 antibody should be determined by the researcher.
• Antibody Type: Primary Antibody
• Clone Number: M130/2163
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Storage: Store the CD163 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Hazard Information: This CD163 antibody is available for research use only.
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

IHC staining of FFPE human tonsil with CD163 antibody (clone M130/2163). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human tonsil with CD163 antibody (clone M130/2163). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human histiocytoma with CD163 antibody (clone M130/2163). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

SDS-PAGE analysis of purified, BSA-free CD163 antibody (clone M130/2163) as confirmation of integrity and purity.