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Catalog Number | orb308750 |
---|---|
Category | Antibodies |
Description | Mouse monoclonal antibody against CD154 conjugated to PE. |
Species/Host | Mouse |
Clonality | Monoclonal |
Clone Number | 24-31 |
Tested applications | FC |
Reactivity | Human, Primate |
Isotype | Mouse IgG1 |
Immunogen | human CD154 fusion protein |
Dilution range | Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests. |
Purity | Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography. |
Conjugation | PE |
Target | CD154 |
Entrez | 21947 |
UniProt ID | P27548 |
Storage | Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze. |
Buffer/Preservatives | Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
Alternative names | anti CD40L antibody Read more... |
Note | For research use only |
Application notes | Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests. |
Expiration Date | 12 months from date of receipt. |
Separation of human CD154 positive lymphocytes (red-filled) from CD154 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human stimulated (PMA + ionomycin) peripheral blood mononuclear cells stained using anti-human CD154 (24-31) PE antibody (10 μl reagent per milion cells in 100 μl of cell suspension).
Flow cytometry surface staining pattern of human stimulated (PMA + ionomycin) peripheral blood mononuclear cells stained using anti-human CD154 (24-31) PE antibody (10 μl reagent per milion cells in 100 μl of cell suspension).
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