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Catalog Number | orb129549 |
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Category | Antibodies |
Description | Rabbit polyclonal antibody to CD133, which is also known as prominin or AC133. CD133 is a marker frequently found on multipotent progenitor cells, including immature hematopoietic stem and progenitor cells. The protein has been extensively used as a stem cell marker for normal and cancerous tissues. |
Clonality | Polyclonal |
Tested applications | ICC, IF, IHC, IP, WB |
Reactivity | Human, Mouse, Rat |
Isotype | IgG |
Immunogen | A synthetic peptide of human CD133(NP_006008.1). |
Concentration | 1 mg/ml |
Dilution range | WB: 1:500-2000, IHC-P: 1:50-100 |
Conjugation | Unconjugated |
MW | 120KDa |
UniProt ID | O43490 |
Storage | Store at -20°C. Avoid freeze / thaw cycles. |
Buffer/Preservatives | PBS with 0.01% thiomersal,50% glycerol,pH7.3. |
Alternative names | anti-AC133 antibody, gen AC133 antibody, anti-CD13 Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
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Singer, Donald et al. Prominin‐1 controls stem cell activation by orchestrating ciliary dynamics The EMBO Journal, e99845 (2018)
Applications
Reactivity
Western blot analysis of extracts of SW620 cell line lysates using CD133 antibody
Immunohistochemical staining of human prostate tissue using CD133 antibody
Immunohistochemical staining of human lung tissue using CD133 antibody
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Staining is not only specific but consistent from lot to lot as well. The recommended dilutions on the data sheet provded are accurate, so no need to do multiple titrations of the antibody.
- Catherine Langford/University of Alabama at Birmingham
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