CD131 Antibody [BION-1], Rabbit IgG

• Catalog Number: orb1671811
• Category: Antibodies
• Description: CD131 Antibody [BION-1], Rabbit IgG
• Clonality: Recombinant
• Clone Number: BION-1
• Tested applications: Blocking, Crystallography, FA, IHC, IP, WB
• Reactivity: Human
• Isotype: IgG kappa
• Immunogen: BALB/c mice were immunized intraperitonally with COS cells transfected DQP (Domain 4 of the common beta-chain) expression constructs. The immunizations were repeated 4 times at 2-week intervals. Four weeks after the final immunization, a mouse was boosted with COS transfectants intravenously. Three days later, splenocytes were harvested and fused with NS-1 myeloma cells. Hybridoma supernatants were screened on CHO DQP cells by flow cytometry, with untransfected CHO cells as a control. All antibodies were from single hybridoma clones as selected by a limiting dilution method. MoAbs were purified from ascites fluid or hybridoma supernatant by a protein A sepharose column.
• Antibody Type: Primary Antibody
• Concentration: batch dependent
• Conjugation: Unconjugated
• Target: CSF2RB
• UniProt ID: P32927
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: CSF2RB, CD131, IL3RB, IL-3R common beta subunit, I
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Flow-cytometry using the Anti-CD131 antibody BION-1. U937 cells were stained with anti-Fluorescein IgG antibody (4-4-20; isotype control - black line) or the r version of BION-1 (orb1671811 - blue line) at a dilution of 1:100 for 1h at RT. After washing- bound antibody was detected using a goat anti-r AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

Immunofluorescence staining of U937 cells using anti-CD131 antibody (BION-1). Immunofluorescence analysis of paraformaldehyde fixed U937 cells immobilized on Shi-fix™ cover-slips and stained with the chimeric r version of BION-1 (orb1671811) at 10 ug/ml followed by Alexa Fluor® 488 secondary antibody (2 ug/ml)- showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom orb1671811- DAPI- merged channels and an isotype control. The isotype control was stained with anti-Fluorescein antibody (3.0) followed by Alexa Fluor® 488 secondary antibody.