CBX1 antibody

• Catalog Number: orb19107
• Category: Antibodies
• Description: Goat polyclonal antibody to CBX1
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Reactivity: Bovine, Canine, Human, Mouse, Rat
• Dilution range: ELISA: 1:32000, WB: 0.3-1.0 μg/ml, IHC-P: 2-3µg/ml
• Conjugation: Unconjugated
• MW: 21.4
• Target: CBX1 / HP1-Beta
• Entrez: 10951
• Protein Sequence: QSQKTAHETDKSE
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti CBX1 antibody, anti HP1-BETA antibody, anti c
• Note: For research use only
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.IHC: In paraffin embedded Human Placenta shows strong nuclear staining in trophoblasts. Recommended concentration, 2-3ug/ml.WB: Approx. 25kDa band observed in lysates of A431, HeLa and HepG2 (calculated MW of 25kDa according to NP_006798.1). Recommended concentration: 0.3-1.0 μg/ml.
• Expiration Date: 12 months from date of receipt.

orb19107 (0.3ug/ml) staining of A431 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

orb19107 (2.5ug/ml) staining of paraffin embedded Human Placenta. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.