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Product Overview
Product Name Caspase 8 antibody
Catalog Number orb10241
Species/Host Rabbit
Reactivity Human, Mouse, Rat
Predicted Reactivity Bovine, Canine, Gallus, Porcine
Conjugation Unconjugated
Tested applications FACS, ICC, IF, IHC-P, P-ELISA
Immunogen KLH conjugated synthetic peptide derived between 421-472 amino acids of human Caspase 8
Target Caspase 8
Alternative Names
Product Properties
Form/Appearance Liquid: 0.01M TBS (pH7.4), 0.5% BSA, 0.015% ProClin™ 300 and 25% glycerol
Concentration - 5 μg (in 100 μl): 0.05 mg/ml
- 20 μg (in 100 μl): 0.2 mg/ml
- 100 μg (in 200 μl): 0.5 mg/ml
- 200 μg (in 400 μl): 0.5 mg/ml
Storage Store at 4°C for up to two weeks. For long term storage, aliquot and store at -20°C, avoid freeze/thaw cycles.
Note For research use only.
Isotype IgG
Clonality Polyclonal
Purity Affinity purified by Protein A
MW 12/55 kDa
Uniprot ID Q14790 (Human); O89110 (Mouse); Q9JHX4 (Rat)
NCBI NP_001073593.11; NM_001080124.11
Entrez 841
Product Description

Rabbit polyclonal antibody to caspase 8 also known as CASP-8 and CAP4 whose expression can be detected in various tissues but is highly expressive in the thymus and spleen. It is found in the CD95 death-inducing signaling complex. Its binding to the adapter molecule FADD recruits it to either receptor. The resulting aggregate called death-inducing signaling complex (DISC) performs CASP8 proteolytic activation. The active dimeric enzyme is then liberated from the DISC and free to activate downstream apoptotic proteases.

Application Notes
Dilution Range P-ELISA: 1:500-1000, IHC-P: 1:400-800μg/Test, IF/ICC: 1:100-500
Validation Images
Immunohistochemical analysis of paraffin embedded human rectal cancer tissue using Caspase 8 antibody
IHC-P of human rectal cancer tissue (Caspase 8 antibody at 1:200)
Immunohistrochemical analysis of formalin fixed and paraffin embedded human rectal carcinoma tissue using Caspase 8 antibody
References

Differential short-term regional effects of early high dose erythropoietin on white matter in preterm lambs after mechanical ventilation.

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