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Product Overview
Product Name CaMKII antibody (ATTO-700)
Catalog Number orb147038
Species/HostMouse
ReactivityRat
ConjugationATTO-700
Tested applicationsELISA, ICC, IF, IHC-P, IP, RIA, WB
Immunogen Partially purified rat CaMKII
Target CaMKII. Detects 50-60kDa protein, corresponding to the molecular mass of CaMKII alpha-subunit on SDS page immunoblots. Recognizes both phosphorylated and non-phosphorylated forms.
Alternative Names
Product Properties
Form/Appearance PBS pH7.4, 50% glycerol, 0.09% sodium azide
Concentration 1 mg/ml
Storage Store at 2-8°C and -20°C for one year. (Shipped on ice packs)
Note For research use only.
Isotype IgG1 Kappa
ClonalityMonoclonal
Purity Protein G Purified
MW 50 kDa
Clone ID 6G9
Uniprot ID P11798
NCBI NP_033922.1
Product Description

Mouse monoclonal to CaMKII (ATTO-700). CaMKII is an important member of calcium/calmodulin- activated protein kinase family, functioning in neural synaptic stimulation and T-cell receptor signaling. CaMKII is expressed in many different tissues but is specifically found in the neurons of the forebrain and its mRNA is found within the dendrites and the soma of the neuron. The CaMKII that is found in the neurons consist of two subunits of 52 (termed alpha genes) and 60 kDa (beta genes). CaMKII has catalytic and regulatory domains, as well as an ATP-binding domain, and a consensus phosphorylation site (3-7). The binding of Ca2+ auto inhibitory effect and activates the kinase. /calmodulin to its regulatory domain releases its This kinase activation results in auto phosphorylation at threonine 286. The threonine phosphorylation state of CaMKII can be regulated through PP1/PKA. Whereas PP1 (protein phosphatase 1) dephosphorylates phospho-CaMKII at Thr286, PKA (protein kinase A) prevents this dephosphorylation. Auto phosphorylation also enables CaMKII to attain an enhanced affinity for NMDA receptors in postsynaptic densities (10-12)..

Application Notes
Application Notes 0.1μg/ml was sufficient for detection of CamKII in 20μg rat brain tissue extract by colorimetric immunoblot analysis using Goat Anti-Mouse IgG:AP as the secondary.
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