CACNA2D2 Antibody

• Catalog Number: orb1269012
• Category: Antibodies
• Description: CACNA2D2 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC-P, WB
• Reactivity: Human, Mouse
• Isotype: Rabbit Ig
• Immunogen: This CACNA2D2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 643-671 amino acids from the Central region of human CACNA2D2.
• Concentration: batch dependent
• Dilution range: For WB starting dilution is: 1:2000For IF starting dilution is: 1:10~50For IHC-P starting dilution is: 1:10~50
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 130 kDa
• Target: CACNA2D2
• UniProt ID: Q9NY47
• NCBI: Q9NY47
• Storage: Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: Supplied in PBS with 0.09% (W/V) sodium azide.
• Alternative names: Voltage-dependent calcium channel subunit alpha-2/
• Note: For research use only
• Application notes: For WB starting dilution is: 1:2000For IF starting dilution is: 1:10~50For IHC-P starting dilution is: 1:10~50
• Expiration Date: 12 months from date of receipt.

Western Blot at 1:2000 dilution + human testis lysate Lysates/proteins at 20 ug per lane.

Western Blot at 1:2000 dilution + mouse kidney lysate Lysates/proteins at 20 ug per lane.

Fluorescent image of U251 cell stained with CACNA2D2 Antibody. U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with CACNA2D2 primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7 units/ml).CACNA2D2 immunoreactivity is localized to Cytoplasm significantly.

CACNA2D2 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human cerebellum tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.