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Catalog Number | orb749617 |
---|---|
Category | Antibodies |
Description | This mAb recognizes a protein of 145kDa, identified as CD117/p145kit/c-Kit. It is found on a wide variety of tumor cells including follicular and papillary carcinoma of thyroid, adenocarcinomas from endometrium, lung, ovary, pancreas, and breast as well as malignant melanoma, endodermal sinus tumor, and small cell carcinoma. However, anti-CD117/c-Kit has been particularly useful in differentiating gastrointestinal stromal tumors from Kaposi's sarcoma, tumors of smooth muscle origin, fibromatosis, and neural tumors of the GI tract. Anti-CD117/c-Kit is also useful in recognizing myeloblasts in bone marrow biopsy and clot section. |
Species/Host | Mouse |
Clonality | Monoclonal |
Clone Number | KIT/983 |
Tested applications | WB |
Reactivity | Human |
Isotype | Mouse IgG1, kappa |
Immunogen | A recombinant human fragment around amino acids 100-300 was used as the immunogen for the c-Kit antibody. |
Dilution range | Western blot: 2-4ug/ml |
Purity | Protein G affinity chromatography |
Conjugation | Unconjugated |
Formula | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide |
Hazard Information | This c-Kit antibody is available for research use only. |
UniProt ID | P10721 |
Storage | Store the c-Kit antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide). |
Buffer/Preservatives | 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide |
Note | For research use only |
Application notes | Optimal dilution of the c-Kit antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min |
Expiration Date | 12 months from date of receipt. |
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