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Product Overview
Product Name BRCA2 antibody
Catalog Number orb10203
Species/HostRabbit
ReactivityHuman, Mouse, Rat
Predicted Reactivity Bovine, Canine, Equine, Gallus
ConjugationUnconjugated
Tested applicationsELISA, IF, IHC-P, WB
Immunogen KLH conjugated synthetic peptide derived between 31-120 amino acids of human BRCA2
Target BRCA2
Alternative Names
Product Properties
Form/Appearance Liquid: 0.01M TBS (pH7.4), 0.5% BSA, 0.015% ProClin™ 300 and 25% glycerol
Concentration - 5 μg (in 100 μl): 0.05 mg/ml
- 20 μg (in 100 μl): 0.2 mg/ml
- 100 μg (in 200 μl): 0.5 mg/ml
- 200 μg (in 400 μl): 0.5 mg/ml
Storage Store at 4°C for up to two weeks. For long term storage, aliquot and store at -20°C, avoid freeze/thaw cycles.
Note For research use only.
Isotype IgG
ClonalityPolyclonal
Purity Affinity purified by Protein A
MW 384 kDa
Uniprot ID P51587 (Human); P97929 (Mouse)
Entrez 675
Product Description

Rabbit polyclonal antibody to BRCA2 which is also known as Fanconi anemia group D1 protein whose expression can be detected in the spleen but it is significantly expressive in the breast and thymus. It is found in the nucleus. It can perform functions like intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator and centrosome duplication. It is extensively involved in double-strand break repair and/or homologous recombination and binds RAD51 and potentiates recombinational DNA repair by promoting assembly of RAD51 onto single-stranded DNA (ssDNA).

Application Notes
Dilution Range WB: 1:500-2000, ELISA: 1:500-1000, IHC-P: 1:400-800, IF: 1:100-500
Validation Images
Immunohistochemical analysis of formalin-fixed and paraffin embedded human breast carcinoma tissue (dilution at:1:200) using BRCA2 antibody
Immunohistochemical analysis of formalin-fixed and paraffin embedded human breast carcinoma tissue (dilution at:1:200) using BRCA2 antibody
References
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