BRAF Mouse Monoclonal Antibody

• Catalog Number: orb1291573
• Category: Antibodies
• Description: BRAF Mouse Monoclonal Antibody
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 3G9
• Tested applications: ELISA, IF, IHC-Fr, IHC-P, IP, WB
• Predicted Reactivity: Mouse, Rat
• Reactivity: Human, Mouse, Rat
• Isotype: IgG2a κ
• Immunogen: Recombinant human B-Raf protein (12-156/766aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:100-1000, IHC-P=1:100-500, IHC-F=1:400-800, IF=1:50-500, ELISA=1:30-3000, IP=1:100-500
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 84(hu)/89(mo, rat) kDa
• Target: BRAF
• UniProt ID: P15056
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: BRAF_HUMAN; Serine/threonine-protein kinase B-raf;
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Sample: Lane 1: Mouse Cerebrum tissue lysates, Lane 2: Mouse Testis tissue lysates, Lane 3: Rat Testis tissue lysates, Lane 4: Human Raji cell lysates, Lane 5: Human Jurkat cell lysates, Lane 6: Human MOLT4 cell lysates, Lane 7: Human HL-60 cell lysates, Primary: Anti-B-Raf (orb1291573) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution, Predicted band size: 84 (hu)/89 (mo, rat) kDa, Observed band size: 95 (hu)/100 (mo, rat) kDa.