BRAF Mouse Monoclonal Antibody

SKU: orb1291573

Description

BRAF Mouse Monoclonal Antibody

Research Area

Cell Biology, Cell Differentiation, Kinases, Signal Transduction, Tyrosine Kinases

Images & Validation

• Tested Applications: ELISA, IF, IHC-Fr, IHC-P, IP, WB
• Dilution Range: WB=1:500-2000, IHC-P=1:50-200, IHC-F=1:50-200, IF=1:50-200, ELISA=1:30-3000, IP=1:100-500
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2a, κ
• Clone No.: B5I1
• Immunogen: Recombinant human B-Raf protein (12-156/766aa)
• Target: BRAF
• Molecular Weight: 95 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

B-RAF1; B-raf; BRAF-1; BRAF1; NS7; RAFB1; 9930012E13Rik; Braf-2; Braf2; C230098H17; D6Ertd631e; BRAF_HUMAN; BRAF; Proto-oncogene B-Raf; p94; v-Raf murine sarcoma viral oncogene homolog B1; 2.7.11.1; BRAF_MOUSE;

Sample: Lane 1: Mouse Cerebrum tissue lysates, Lane 2: Mouse Testis tissue lysates, Lane 3: Rat Testis tissue lysates, Lane 4: Human Raji cell lysates, Lane 5: Human Jurkat cell lysates, Lane 6: Human MOLT4 cell lysates, Lane 7: Human HL-60 cell lysates, Primary: Anti-B-Raf (orb1291573) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution, Predicted band size: 84 (hu)/89 (mo, rat) kDa, Observed band size: 95 (hu)/100 (mo, rat) kDa.

Quick Database Links

Gene Symbol

BRAF

UniProt

P15056