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Product Overview
Product Name BPG 13-step Protein Ladder
Catalog Number orb90563
Tested applications SDS-PAGE, WB
Hazard Information Non-Toxic
Target Protein
Alternative Names
Product Properties
Form/Appearance Approximately 0.1-0.4 mg/ml of each protein in the buffer (20 mM Tris-phosphate, pH 7.5 at 25°C), 2 % SDS, 10 mM Dithiothreitol, 3.6 M Urea, and 15 % (v/v) Glycerol).
Storage Stable for up to 3 months at 4°C. For long term storage, store at -20°C.
Note For research use only.
Product Description

The BPG 13-step Protein Ladder is a three-color protein standard with 13 pre-stained proteins covering a wide range molecular weights from 3.5 to 245 kDa. Proteins are covalently coupled with a blue chromophore except for two reference bands (one green and one red band at 25 kDa and 75 kDa respectively) when separated on SDS-PAGE (Tris-glycine buffer).

Application Notes
Application Notes Experiment Notes:

1. 3 μl or 5 μl per loading for clear visualization during electrophoresis on 15-well or 10-well mini-gel, respectively.

2. 1.5-2.5 μl per well for general Western transferring.

3. Apply more for thicker (> 1.5 mm) or larger gel.

Validation Images
"Migration patterns of BPG 13-step Protein Ladder in different electrophoresis conditions."
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