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| Catalog Number | orb782480 |
|---|---|
| Category | Assays and Kits |
| Description | This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with 17-Hydroxyprogesterone(17-OHP) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to 17-Hydroxyprogesterone(17-OHP). |
| Tested applications | ELISA |
| Reactivity | Bovine |
| Range | 312.5-20000 pg/mL |
| Concentration | 20000 pg/mL |
| Sensitivity | 88.5 pg/mL |
| Protein Length | 2h |
| Alternative names | 17OHP ELISA Kit, 17-OH Progesterone ELISA Kit, 17- Read more... |
| Note | For research use only |
| Application notes | standard: 20000 pg/mL. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Cattle 17-OHP protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cattle 17-OHP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cattle 17-OHP in the samples is then determined by comparing the OD of the samples to the standard curve |
| Sample Types | serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
| Expiration Date | Please enquire. |
