BirA antibody

• Catalog Number: orb750518
• Category: Antibodies
• Description: BirA antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Isotype: Antiserum
• Immunogen: Anti-Biotin Ligase Epitope Tag antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic avi-tag peptide GLNDIFEAQKIEWH (minimal BirA substrate; biotin is added to the sequence at the lysine).
• Concentration: 70 mg/ml
• Dilution range: ELISA: 1:5,000, WB: 1:5,000
• Form/Appearance: Liquid (sterile filtered)
• Purity: This antiserum detects the minimal peptide substrate (GLNDIFEAQKIEWH) for the E. coli biotin ligase tag (BLT of BirA). Western blot analysis of B. subtilis strains producing either (BAP-tagged) SPoIIIAH (XWB49) or (BAP-tagged) SpoIIQ (XWB46) showed strong and specific reactivity to the antisera. Minor background bands of unknown identity may be observed. The antisera will recognize the BAP/BLT tag irrespective of the whether the tag is biotinylated (data not shown).
• Conjugation: Unconjugated
• Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: rabbit anti-Biotin Ligase Epitope Tag Antibody, ra
• Note: For research use only
• Application notes: Anti-Biotin Ligase Epitope Tag Antibody has been tested in ELISA and Western Blot. Western blot analysis of B. subtilis strains producing either (BAP-tagged) SPoIIIAH (XWB49) or (BAP-tagged) SpoIIQ (XWB46) showed strong and specific reactivity to the antisera. Minor background bands of unknown identity may be observed. The antisera will recognize the BAP/BLT tag irrespective of the whether the tag is biotinylated (data not shown).
• Expiration Date: 12 months from date of receipt.