Catalog Number: orb64051

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Form/Appearancefiltered solution (30 kDa) in water. pH: 7.5 ±0.5.
Catalog Numberorb64051
FormulaC32H52N7O18P3S (free acid)

≥ 98 % (HPLC)

MW947.78 g/mol (free acid)
Application notes

Incorporation into DNA/cDNA by
- PCR with Taq polymerase [1,2] & in-house data
- Nick Translation with DNAse I/ DNA Polymerase I [3,4] & in-house data
- Primer Extension with Klenow exo- [2,5]
- 3’-End Labeling with Terminal deoxynucleotidyl Transferase (TdT) [6,7]
- Reverse Transcription with MMLV Reverse Transcriptase [8,9]
Incorporation into RNA by
- 3’-End Labeling with Terminal deoxynucleotidyl Transferase (TdT) [10]

NoteFor research use only.

1.0 mM - 1.1 mM

Hazard InformationNon-Toxic
Storagestore at -20 °C
Expiration Date12 months from date of receipt.
DescriptionBiotin-16-dUTP is enzymatically incorporated into DNA/cDNA as substitute for its natural counterpart dTTP. The resulting Biotin-labeled DNA/cDNA probes are subsequently detected using streptavidin conjugated with horseradish peroxidase (HRP), alkaline phosphatase (AP), a fluorescent dye or agarose/magnetic beads. Optimal substrate properties and thus labeling efficiency as well as an efficient detection of the Biotin moiety is ensured by a 16-atom linker attached to the C5 position of uridine. Recommended Biotin-16-dUTP/dTTP ratio for PCR and Nick Translation: 50% Biotin-16-dUTP/ 50% dTTP
Structural formula of Biotin-16-dUTP
Structural formula of Biotin-16-dUTP
  1. New
    Biotin-16-dUTP [orb1690206]





    5 mg