Biotin-16-dUTP

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Form/Appearance	filtered solution (30 kDa) in water. pH: 7.5 ±0.5.
Catalog Number	orb64051
Formula	C32H52N7O18P3S (free acid)
Purity	≥ 98 % (HPLC)
MW	947.78 g/mol (free acid)
Application notes	Applications: Incorporation into DNA/cDNA by - PCR with Taq polymerase ^{[1,2] & in-house data} - Nick Translation with DNAse I/ DNA Polymerase I ^{[3,4] & in-house data} - Primer Extension with Klenow exo^- ^[2,5] - 3’-End Labeling with Terminal deoxynucleotidyl Transferase (TdT) ^[6,7] - Reverse Transcription with MMLV Reverse Transcriptase ^[8,9] Incorporation into RNA by - 3’-End Labeling with Terminal deoxynucleotidyl Transferase (TdT) ^[10]
Note	For research use only.
Concentration	1.0 mM - 1.1 mM
Hazard Information	Non-Toxic
Storage	store at -20 °C
Expiration Date	12 months from date of receipt.
Description	Biotin-16-dUTP is enzymatically incorporated into DNA/cDNA as substitute for its natural counterpart dTTP. The resulting Biotin-labeled DNA/cDNA probes are subsequently detected using streptavidin conjugated with horseradish peroxidase (HRP), alkaline phosphatase (AP), a fluorescent dye or agarose/magnetic beads. Optimal substrate properties and thus labeling efficiency as well as an efficient detection of the Biotin moiety is ensured by a 16-atom linker attached to the C5 position of uridine. Recommended Biotin-16-dUTP/dTTP ratio for PCR and Nick Translation: 50% Biotin-16-dUTP/ 50% dTTP

Structural formula of Biotin-16-dUTP